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KMID : 0620920080400050495
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Experimental & Molecular Medicine
2008 Volume.40 No. 5 p.495 ~ p.504
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Gene expression profiling of light-induced retinal degeneration in phototransduction gene knockout mice
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Jayalakshmi Krishnan
Choi Sang-Dun
Jiayan Chen
Shin Kum-Joo
Hwang Jong-Ik
Han Sang-Uk
Lee Gwang
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Abstract
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Exposure to light can induce photoreceptor cell death and exacerbate retinal degeneration. In this study, mice with genetic knockout of several genes, including rhodopsin kinase (Rhok-/-), arrestin (Sag-/-), transducin (Gnat1-/-), c-Fos (c-Fos-/-) and arrestin/transducin (Sag-/-/Gnat1-/-), were examined. We measured the expression levels of thousands of genes in order to investigate their roles in phototransduction signaling in light-induced retinal degeneration using DNA microarray technology and then further explored the gene network using pathway analysis tools. Several cascades of gene components were induced or inhibited as a result of corresponding gene knockout under specific light conditions. Transducin deletion blocked the apoptotic signaling induced by exposure to low light conditions, and it did not require c-Fos/AP-1. Deletion of c-Fos blocked the apoptotic signaling induced by exposure to high intensity light. In the present study, we identified many gene transcripts that are essential for the initiation of light-induced rod degeneration and proposed several important networks that are involved in pro- and anti-apoptotic signaling. We also demonstrated the different cascades of gene components that participate in apoptotic signaling under specific light conditions.
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KEYWORD
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arrestin, gene expression profiling, oligonucleotide arrestin, gene expression profiling, oligonucleotide proteins c-fos, retinal degeneration, transducin
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